Calcifying odontogenic cyst with AOT-like features: a case report and literature review / Cisto odontogênico calcificante com características adenomatóides: relato de caso e revisão de literatura

Odontogenic lesions are a heterogeneous group of diseases that presents differences in their biological behavior and the occurrence of variable inductive interactions. Calcifying odontogenic cyst (COC), or Gorlin's cyst, is a well-recognized example of these lesions. We describe a case of COC with AOT-like areas and highlights its morphological diversity. A 60-year-old pheoderma man presented with a large swelling in the anterior buccal region of the mandible. Panoramic radiography revealed a well-defined, unilocular, radiolucent lesion associated with important root resorption. Complete enucleation of the lesion was performed and the histopathological findings met the criteria for the diagnosis of COC, although the cyst exhibited unusual AOT-like features. The patient has been recurrence free for 6 months after surgery. COCs with AOT-like features are rare, and reflect the multipotentiality and complexity of the inductive effects of the odontogenic epithelium with the ectomesenchyme. Enucleation seems to be the most indicated treatment, similar to classical COC (AU)

As lesões odontogênicas são um grupo heterogêneo de patologias que apresentam diferenças no seu comportamento biológico, e ocorrência de interações indutivas variáveis. O cisto odontogênico calcificante (COC), ou cisto de Gorlin, é um exemplo bem conhecido destas lesões. Descrevemos um caso de COC com áreas adenomatóides e destacamos a sua diversidade morfológica. Paciente do sexo masculino, 60 anos de idade, apresentou um aumento de volume na região anterior da mandíbula. A radiografia panorâmica revelou uma lesão bem definida, unilocular e radiolúcida associada a uma reabsorção radicular importante. A enucleação completa da lesão foi realizada e os achados histopatológicos preencheram os critérios para o diagnóstico de COC, embora o cisto exibisse características adenomatóides pouco usuais. O paciente permanece livre de recidivas durante 6 meses após a cirurgia. Os COCs com características adenomatóides são raros, e refletem a multipotencialidade e complexidade dos efeitos indutivos do epitélio odontogênico com o ectomesênquima. A enucleação parece ser o tratamento mais indicado, semelhante ao COC clássico. (AU)

Tumor Odontogénico Adenomatoide Quístico, presentación inusual. Reporte de dos casos

RESUMEN: El tumor odontogénico adenomatoide es un tumor benigno, poco común, que corresponde al 2-7% de los tumores odontogénicos. Se presenta con mayor prevalencia en la segunda década de vida y se ubica preferentemente en maxilar anterior. Es una lesión encapsulada, no agresiva, de crecimiento lento, que puede pasar desapercibida durante años. Deriva del epitelio odontogénico, y se caracteriza histológicamente por células epiteliales dispuestas en nidos, remolinos o cordones con escaso tejido conjuntivo. Puede presentar estructuras canaliculares un patrón ductiforme, con calcificaciones y presenta cápsula. El presente estudio reporta dos casos, el primero es una mujer de 30 años y el segundo el de un hombre de 36 años, ambos con un tumor odontogénico adenomatoide ubicado en zona maxilar anterior derecha, asintomático, sin asociación con diente incluido y de presentación quística a nivel imagenológico e histopatológico. Se discuten características clínicas, radiográficas, histopatológicas y terapéuticas de los casos.

ABSTRACT: Adenomatoid odontogenic tumor is a benign, rare tumor that accounts for 2-7% of odontogenic tumors. It appears with greater prevalence in the second decade of life and is located preferentially in the anterior maxilla. It is a slow-growing, non-aggressive, encapsulated lesion that can go unnoticed for years. It is derived from the odontogenic epithelium, and is characterized histologically by epithelial cells arranged in nests, eddies, or cords with little connective tissue. It may have canalicular structures and a ductiform pattern, with calcifications and it has a capsule. The present study reports two cases, the first is a 30-year-old woman and the second is a 36-year-old man, both with an adenomatoid odontogenic tumor located in the right anterior maxillary area, asymptomatic, without association with an included tooth and cystic presentation at the imaging and histopathological levels. The clinical, radiographic, histopathological and therapeutic characteristics of the cases are discussed.

Tumor odontogénico adenomatoide en dos adolescentes / Adenomatoid odontogenic tumor in two adolescents

Se describen 2 casos clínicos de adolescentes de ambos sexos, quienes presentaron edemas en el lado izquierdo de la mandíbula con reabsorción dental y en la porción anterior del maxilar, respectivamente. La histopatología reveló una variante folicular intraósea del tumor odontogénico adenomatoide. El tratamiento quirúrgico empleado fue una enucleación con resultados satisfactorios.

Two cases reports of adolescents from both sexes are described, they presented edemas in the left side of the maxillary with dental reabsorption and in the anterior portion of the maxillary, respectively. The histopathology revealed an intraosseous folicular variety of the adenomatoid odontogenic tumor. The surgical treatment used was an enucleation with satisfactory results.

Tumor odontogénico adenomatoide extrafolicular de localización mandibular / Mandibular extrafollicular adenomatoid odontogenic tumor

Introducción: el tumor odontogénico adenomatoide es un tumor odontogénico benigno compuesto por epitelio odontogénico con estroma fibroso maduro sin participación del ectomesénquima. Representa entre el 2 por ciento y el 7 por ciento de estos tumores. Más del 90 por ciento aparecen antes de los 30 años con tres variantes clínicas: folicular, extrafolicular y periférica. Su aspecto clínico-radiográfico varía y puede ser confundido con otras lesiones quísticas o neoplásicas de la cavidad bucal, por lo que es necesario el diagnóstico anatomopatológico. Objetivo: presentar un caso de un tumor odontogénico adenomatoide extrafolicular mandibular. Presentación del caso: paciente femenina de 12 años de edad, con aumento de volumen asintomático en la encía mandibular del lado izquierdo, sin antecedentes patológicos de interés, de tiempo de evolución no precisado. Al examen físico se observó aumento de volumen de forma redondeada de 1,5 cm que desplazaba la encía mandibular izquierda entre incisivo lateral y canino. La radiografía reveló una lesión radiolúcida unilocular entre 42 y 43 que expandía la cortical ósea. Se detectó ausencia de vitalidad pulpar de estos dientes. Con el diagnóstico clínico de quiste periapical se realizó excisión quirúrgica, se envió la muestra al laboratorio de Anatomía Patológica, y se concluyó el diagnóstico de tumor odontogénico adenomatoide extrafolicular. Conclusiones: el tumor odontogénico adenomatoide, más común en la maxila, puede presentarse en la mandíbula. Los hallazgos clínico-radiográficos semejan otras lesiones odontogénicas como quistes dentígeros, otras neoplasias y lesiones periapicales inflamatorias, siendo el diagnóstico anatomopatológico el concluyente(AU)

Introduction: adenomatoid odontogenic tumor is a benign odontogenic tumor composed of odontogenic epithelium with mature fibrous stroma without ectomesenchymal involvement. It represents between 2 percent and 7 percent of these tumors. More than 90 percent appear before age 30, with three clinical variants: follicular, extrafollicular and peripheral. Its clinical-radiographic aspect may vary, and it may be confused with other cystic or neoplastic lesions of the oral cavity, hence the need for an anatomo-pathological diagnosis. Objective: present a case of mandibular extrafollicular adenomatoid odontogenic tumor. Case presentation: afemale 12-year-old patient presents with asymptomatic left mandibular gum swelling, with no pathological antecedents of interest and an imprecise time of evolution. Physical examination found a round 1.5 cm swelling displacing the left mandibular gum between the lateral incisor and the canine. Radiography revealed a unilocular radiolucent lesion between teeth 42 and 43 expanding the cortical bone. The teeth involved showed no pulpal vitality. Upon reaching a clinical diagnosis of periapical cyst, surgical excision was performed and a sample was submitted to the Anatomical Pathology laboratory. The anatomo-pathological diagnosis was extrafollicular adenomatoid odontogenic tumor. Conclusions: though more common in the maxilla, adenomatoid odontogenic tumors may also occur in the mandible. Clinical and radiological features may be similar to those of other odontogenic lesions, such as dentigerous cysts, other neoplasms and periapical inflammatory lesions; therefore, the final diagnosis should be provided by anatomo-pathological evaluation(AU)

Epithelial odontogenic tumors: analysis of 156 cases in a Brazilian population / Tumores odontogénicos epiteliales: análisis de 156 casos en una población brasileña

The objective of this study was to determine the distribution of epithelial odontogenic tumors diagnosed histologically in a period of 41 years in a Brazilian population according to age, gender, site affected and compare these data with previously reported studies from other countries. Data of epithelial odontogenic tumors diagnosed were collected from the files of the Oral Pathology Laboratory of Federal University of Rio Grande do Norte, Natal, RN, Brazil, and entered in a standardized form for later comparisons. Clini-cal features obtained from the patient records and microscope slides were reviewed according to the 1992 World Health Organization classification. A total 156 epithelial odontogenic tumor were reported. Of these, all of them were benign. Ameloblastoma was the most frequent type (85.9 %), followed by adenomatoid odontogenic tumor (10.9 %) and calcifying epithelial odontogenic tumor (3.2 %). The mean age of the patients was 38 years, with a wide range (11­80 years). The posterior region of mandible was the anatomic site most frequently affected by this disease, and no significant differences were found between sexes in the diagnosis of odontogenic tumors. A marked geographic variation in the relative incidences of various epithelial odontogenic tumors was found. It was particularly notable in ameloblastomas and adenomatoid odontogenic tumors, with the incidences observed in the present study being similar, sometimes different to earlier studies in others parts of the world.

El objetivo fue determinar la distribución de los tumores odontogénicos epiteliales diagnosticados histológicamente en un período de 41 años en una población brasileña según edad, sexo y la zona afectada y comparar estos datos con estudios anteriores de otros países. Los datos de los tumores odontogénicos epiteliales diagnosticados fueron obtenidos de los archivos del Laboratorio de Patología Oral de la Universidad Federal de Rio Grande do Norte, Natal, RN, Brasil, e introducidos en un formulario estandarizado para comparaciones futuras. Las características clínicas obtenidas a partir de los registros de los pacientes y los portaobjetos de microscopio fueron revisados de acuerdo a la clasificación de la Organización Mundial de la Salud 1992. Se informó de un total de 156 tumores epiteliales odontogénicas. De estos, todos eran benignos. Ameloblastoma fue el tipo más frecuente (85,9 %), seguido por el tumor odontogénico adenomatoide (10,9 %) y el tumor odontogénico epitelial calcificante (3,2 %). La edad media de los pacientes fue de 38 años, con un rango amplio (11­80 años). La región posterior de la mandíbula era el sitio anatómico más afectado por esta enfermedad, y no se encontraron diferencias significativas entre sexos en el diagnóstico de los tumores odontogénicos. Se encontró una marcada variación geográfica en las incidencias relativas de diversos tumores odontogénicos epiteliales. Fue particularmente notable en ameloblastomas y tumores odontogénicos adenomatoide, con las incidencias observadas en este estudio siendo a veces similares, y a veces diferentes de los estudios anteriores en otras partes del mundo.

Cell cycle aberration in ameloblastoma and adenomatoid odontogenic tumor: As evidenced by the expression of p53 and survivin.

Context: p53 and survivin are involved in cell cycle progression and inhibition of apoptosis, respectively. Survivin is a unique protein which functions in progression of cell division and inhibits apoptosis leading to cell proliferation and cell survival. According to the literature, mutation of p53 leads to promotion of survivin function. Thus, the importance of cell cycle aberration and uncontrolled proliferation resulting from mutation of p53 and up‑regulation of survivin is discussed. Aims: To assess the role of p53 and survivin in ameloblastoma and adenomatoid odontogenic tumor (AOT). Settings and Design: The percentages of positive tumor cells were considered for statistical evaluation. Nuclear labeling index for p53 and nuclear, cytoplasmic and combined labeling index for survivin was obtained from the stained slides. Materials and Methods: Immunohistochemical expression of p53 and survivin was done qualitatively and quantitatively in 25 cases each of ameloblastoma and AOT. Statistical Analysis Used: Mann–Whitney U‑test, Wilcoxon signed ranks test and Pearson’s correlation test. Results: Quantitatively, p53 and survivin expression was statistically significant in AOT (P = 0.003) and qualitatively, in ameloblastoma (P = 0.004). Survivin expression was significant (P = 0.002) between the study groups unlike that of p53 (P = 0.554). Conclusions: There was no much difference in p53 expression in ameloblastoma and AOT suggestive of cell cycle aberration in both the odontogenic tumors, but significant difference in survivin expression in ameloblastoma and AOT with higher percentage of positive cells in ameloblastoma may be indicative of an aggressive behavior of ameloblastoma.

Adenomatoid Odontogenic Tumor: An Unusual Presentation.

Adenomatoid odontogenic tumor (AOT) is a not so common neoplasm of the odontogenic origin, accounting for around 3-7% of all odontogenic tumors. It is usually present in young females in the anterior maxillary region and thus commonly referred to as a two-third tumor. It is a benign (hamartomatous), usually non-invasive tumor which shows slow and progressive growth. Due to its rarity and varied clinical presentation it is often misdiagnosed. To add to confusion further, there are various lesions which mimic AOT closely. Herein this article, we present a case report of a young female patient with an unusual presentation of AOT tumor in the mandibular region focusing on the versatility of this rare entity.

Tumor odontogénico adenomatoide. Reporte de un caso y revisión de la literatura / Adenomatoid odontogenic tumor. Case report and literature review

El tumor odontogénico adenomatoide es una neoplasia de tipo benigno hamartomatosa de origen odontogénico, se presenta en pacientes jóvenes, siendo el género femenino y la región maxilar los más afectados, de crecimiento lento, asintomáticos, se relacionan a órganos dentarios no erupcionados principalmente el canino, clínicamente se clasifica como: folicular, extrafolicular y periférico, el tratamiento para esta lesión es la enucleación y curetaje de la zona afectada sin observarse recidiva.

Adenomatoid odontogenic tumor is a hamartomous benign neoplasia of odontogenic origin. It appears mostly in young patients and females, the maxillary region being the most affected. It is a slow-growing, asymptomatic lesion. It is related to non-erupted teeth, mainly canines. Lesions of this type can be clinically classified as a follicular, extra follicular and peripheral lesions. Treatment for these lesions is enucleation and curettage of affected area. No recurrence has been observed.

Adenomatoid Odontogenic Tumor: A Case Report.

Adenomatoid odontogenic tumor (AOT) is a very rare odontogenic tumor with an incidence of 1%. Overall it accounts for 9% of all odontogenic tumors. In most of the cases, AOT is misdiagnosed as an odontogenic cyst. Younger individuals are commonly affected and particularly in females. AOT is seen predominantly in the maxillary anterior region in association with an unerupted tooth. Permanent dentition is affected more than the deciduous dentition. Intraoral periapical radiographs play a major role in the diagnosis compared to orthopantomogram because of its increased contrast but for the better assessment of the extension of larger lesions orthopantomogram is must. AOT resembles benign odontogenic lesions like dentigerous cyst and tumors like ameloblastoma. The lesions are managed conservatively by surgical excision along with the removal of the affected tooth and have an excellent prognosis.With this background, we report an unusual case of AOT involving maxillary anterior region in 15-year-old male patient. The present article reviews the etiology, clinical features, histopathological features, and treatment modalities of AOT.

Adenomatoid odontogenic tumor associated with an unerupted mandibular lateral incisor: a case report

Adenomatoid odontogenic tumor (AOT) is a rare, benign odontogenic tumor that predominantly appears in the second decade of life in female patients. Most AOTs occur in the anterior part of the maxilla and are usually associated with impacted anterior teeth. There are three types of AOT, follicular, extrafollicular, and peripheral, which are classified based on the location of the lesion and its association with the impacted tooth. We report a rare case of AOT associated with an impacted right mandibular lateral incisor in an 11-year-old female patient.

Adenomatoid odontogenic tumor with clear cell changes.

Adenomatoid odontogenic tumor (AOT) has a limited biological profi le and been an attention-grabbing tumor for a century for its origin. Though described earlier, it was widely accepted after Harbitz from Norway reported about this uncommon benign tumor in 1915. There has been a long debate as whether this tumor is a hamartoma or a neoplasm. Here, we present a case of AOT in a 20-year-old female with details of clinical, radiological and histological features along with clear cell changes, signifying AOT to be more aggressive in nature than assessed from earlier literature. Thus, we did an extensive search of PubMed literature on AOT with all its histopathological features associated until date to fi nd the report of clear cell changes yet.

Follicular Adenomatoid Odontogenic Tumor.

Adenomatoid odontogenic tumor is an uncommon odontogenic lesion, composed of odontogenic epithelium, characterized histologically by duct like structures with amyloid like deposits, noninvasive lesion with slow but progressive growth. Here we are reporting a case of adenomatoid odontogenic tumor in a 16-year-old female patient in the maxillary region. This paper provides the controversies regarding its origin and management in light of recent findings, clinical, radiographic, histopathologic and therapeutic features of the adenomatoid odontogenic tumor.

Current Concepts and Occurrence of Epithelial Odontogenic Tumors: I. Ameloblastoma and Adenomatoid Odontogenic Tumor

Ameloblastomas and adenomatoid odontogenic tumors (AOTs) are common epithelial tumors of odontogenic origin. Ameloblastomas are clinico-pathologically classified into solid/multicystic, unicystic, desmoplastic, and peripheral types, and also divided into follicular, plexiform, acanthomatous, granular types, etc., based on their histological features. Craniopharyngiomas, derived from the remnants of Rathke's pouch or a misplaced enamel organ, are also comparable to the odontogenic tumors. The malignant transformation of ameloblastomas results in the formation of ameloblastic carcinomas and malignant ameloblastomas depending on cytological dysplasia and metastasis, respectively. AOTs are classified into follicular, extrafollicular, and peripheral types. Ameloblastomas are common, have an aggressive behavior and recurrent course, and are rarely metastatic, while AOTs are hamartomatous benign lesions derived from the complex system of the dental lamina or its remnants. With advances in the elucidation of molecular signaling mechanisms in cells, the cytodifferentiation of epithelial tumor cells in ameloblastomas and AOTs can be identified using different biomarkers. Therefore, it is suggested that comprehensive pathological observation including molecular genetic information can provide a more reliable differential diagnosis for the propagation and prognosis of ameloblastomas and AOTs. This study aimed to review the current concepts of ameloblastomas and AOTs and to discuss their clinico-pathological features relevant to tumorigenesis and prognosis.

Adenomatoid odontogenic tumor of the mandible with unusual radiographic features: A case report

Adenomatoid odontogenic tumor (AOT) usually presents as a unilocular, pericoronal radiolucency in the maxillary anterior region in adolescent females. Very few conditions occur in such a narrow age range and at such a restrictive site. Rarely, these tumors present with varied clinical features. A case of AOT of the mandible is reported with unusual features such as large size, multilocular appearance, and aggressive behavior. The role of radiology in diagnosis of atypical AOT is extremely important. The unique radiological manifestations of the lesion helped in the diagnosis, and it was managed conservatively with no evidence of recurrence.

Tumor odontogênico adenomatóide em mandíbula / Tumor odontógeno adenomatoide de la mandíbula / Adenomatoid odontogenic tumor in mandible

El tumor odontógeno adenomatoide es una lesión relativamente infrecuente que afecta principalmente a las mujeres durante la segunda década de vida, porque su sitio predilecto es la región anterior de la mandíbula, con una lesión por lo general asociada con la corona del diente. Se reporta un caso de tumor odontógeno adenomatoide en la región anterior de la mandíbula junto con sus resultados clínicos, radiológicos e histológicos así como su tratamiento quirúrgico(AU)

Adenomatoid odontogenic tumor is a relatively uncommon lesion, which affects mainly individuals of the female during the second decade due, showing as a site of predilection for the anterior region of the maxilla presenting lesion usually associated with the crown of a tooth incluso. Os authors report a case of an adenomatoid odontogenic tumor in the anterior region of mandible, together with their clinical, radiographic and histological and its surgical treatment(AU)

Tumor odontogênico adenomatóide é uma lesão relativamente incomum, que acomete preferencialmente indivíduos do sexo feminino durante a segunda década de vida. Exibe como sítio de predileção a região anterior da maxila, é geralmente associado à coroa de um dente incluso. Este trabalho tem o objetivo de apresentar um caso clínico de tumor odontogênico adenomatóide. Este se apresenta localizado em região anterior da mandíbula. Pretende-se ainda abordar suas características clínicas, radiográficas e histológicas, além do tratamento cirúrgico conservador de eleição(AU)

Tumor odontogénico adenomatoide en maxilar: reporte de un caso y revisiónde la literatura / Adenomatoid odontogenic tumor in maxilla: a report case and review of the literature

El tumor odontogénico adenomatoide es una neoplasia benigna según la nueva clasificación del 2005 sobre tumores odontogénicos. Es una lesión muchas veces de crecimiento lento pero progresivo y no invasivo. Este reporte describe una paciente de 17 años, sexo femenino, con un tumor odontogénico adenomatoide del subtipo folicular en la región maxilar anterior comprometiendo al canino maxilar izquierdo. Además se realiza una revisión para los profesionales de la salud sobre aspectos diagnósticos y manejo según los hallazgos más recientes en esta patología.

Adenomatoid odontogenic tumor is a benign neoplasm in the new classification of odontogenic tumors of 2005. This lesion is often slow growing but progressive and noninvasive. This report describes a 17-year old female patient with an adenomatoid odontogenic tumor follicular subtype in the anterior maxillary region compromising the left maxillary canine. This article also provides a refresher for the health professionals on its diagnosis and management according to recent findings in this pathology.

Combined Adenomatoid Odontogenic Tumor and Calcifying Epithelial Odontogenic Tumor in the Mandible: Case Report

Adenomatoid odontogenic tumor with rare clinical and radiological presentation – a case report

The Adenomatoid odontogenic tumor (AOT) is a benign (hamartomatous) noninvasive lesion but progressive growth, constituting only 3% of all odontogenic tumors. Most common site of AOT is maxillary anterior region especially canine region. Most common variety of AOT is Follicular variety (73%) which is associated with impacted tooth (maxillary canine). Females are most commonly affected than males. We report on a rare case of follicular AOT in the mandibular anterior region seen in an 18 years old male patient. Diagnosis of adenomatoid odontogenic tumor should be considered when the clinician is presented with a corticated radiolucency in the anterior lower jaw, especially in teens and young adults.

Tumor odontógeno adenomatoide en región mandibular / Adenomatoid odontogenic tumor in mandibular region

El tumor odontogénico adenomatoide es un tumor poco frecuente derivado del epitelio odontontogénico, que contiene estructuras canaliculares con modificaciones inductivas de intensidad variable en el tejido conjuntivo. Es una lesión de crecimiento lento y poco invasiva pero que se puede asemejar a otras lesiones odontógenas de mayor agresividad como el quiste dentígero y el ameloblastoma entre otros. Su localización clásica (área de caninos superiores) nos orienta al diagnóstico y su patrón histológico ductiforme es muy propio de este tumor. Otros tumores que se encuentran dentro de este grupo son el fibroma ameloblástico, el odontoameloblastoma, el quiste odontógeno calcificante y los odontomas compuesto y complejo. Este grupo de lesiones puede o no tener formaciones de tejido duro dental dentro de ellos. Por esta razón, se presenta un paciente con este tipo de tumor, al que se le realizó estudio histopatológico, se revisó la literatura acerca de este tumor odontogénico benigno y sus características clínicas, radiográficas, tratamiento, así como los diagnósticos diferenciales que se deben tener en cuenta(AU)

The adenomatoid odontogenic tumor is an uncommon neoplasm derivative of the odontogenic epithelium containing canalicular structures with inductor modifications of variable intensity in the conjunctival tissue. It is a slow growth lesion and no much invasive but that may to be similar to other odontogenic lesions more aggressive including the dentigerous cyst and the ameloblastoma among others. Its classical location (upper canine area) guides us to diagnosis and its duct histological pattern is very typical of this tumor. Other tumors included in this group are the ameloblastic fibroma, the ameloblastic odontoma, the calcified odontogenic cyst and composed and complex odontomas. This group of lesions may or not to have formations of hard tissue inside. Thus, authors present the case of a patient presenting with this type of tumor making a histopathology study, a literature review on this benign odontogenic tumor and its clinical radiographic features, treatment, as well as the differential diagnoses to be into account(AU)

Análise imuno-histoquímica das citoqueratinas em ameloblastoma e tumor odontogênico adenomatóide / Immunohistochemical analysis of cytokeratins in ameloblastoma and adenomatoid odontogenic tumor

OBJETIVO: O presente trabalho teve por objetivo traçar o perfil das citoqueratinas (CKs) 7, 8, 10, 13, 14, 18 e 19 em ameloblastomas e tumor odontogênico adenomatóide (TOA) visando contribuir para o entendimento da histogênese desses tumores e somar com os resultados já relatados na literatura. MATERIAL E MÉTODO: do arquivo do Laboratório de Anatomia Patológica do Departamento de Odontologia da Universidade Federal do Rio Grande do Norte (UFRN) foi selecionada uma amostra com dez casos de ameloblastomas e oito de TOA para o estudo imuno-histoquímico, utilizando-se anticorpos anti-CKs pelo método da estreptoavidina-biotina. RESULTADOS: Observou-se que nos ameloblastomas a CK 14 esteve presente em todos os casos, enquanto a CK 19 foi observada nas células periféricas (oito casos) e nas centrais (cinco casos). Para os TOA, observou-se imunopositividade para a CK 14 em todos os casos, enquanto a CK 19 esteve marcada predominantemente nas células ductais (seis casos). CONCLUSÃO: As citoqueratinas são expressas de forma variada nos ameloblastomas e nos TOA, os quais preservam CK típicas do germe dental em estágios avançados do desenvolvimento, confirmando sua origem exclusiva a partir do epitélio odontogênico e não se evidenciando CK características do epitélio escamoso.

OBJECTIVES: The aim of the present study was to describe the immunohistochemical expression of cytokeratins (CKs) 7, 8, 10,13, 14, 18 and 19 in the epithelial components of ameloblastomas and adenomatoid odontogenic tumor (AOT). The results were compared and histogenesis discussed. MATERIAL AND METHOD: Specimens of ten ameloblastomas and eight adenomatoid odontogenic tumors were examined by immunohistochemistry using streptavidin-biotin-peroxidase complex method and anti-CKs antibody. The sample was obtained from Department of Oral Pathology, Federal University of Rio Grande do Norte. RESULTS: Immunohistochemical reactivity for CK14 was detected in all cases of ameloblastomas, and the expression of CK19 was diffusely present in peripheral cells (eight cases) and in central cells of five ameloblastomas. In AOTs, immunopositivity for CK14 was detected in all cases, whereas CK 19 was predominantly expressed in ductal cells (six cases). CONCLUSION: The CKs was expressed by several forms in ameloblastomas and AOTs, that have shown typical CKs of dental germ suggesting that these tumors have odontogenic epitheilial differentiation, whereas it has not evinced proper CKs of squamous epithelial.